This procedure describes a so called honey tek which can be used to grow stock liquid mycelium culture used to innoculate new growing media.

 This tek can be uaed to multiply isolated strains grown on agar plates and cloning fresh mushroom flesh by cutting a little piece from the inner flesh and transfer that into the liquid growing medium.

 This tek is a log/description of my experiments in avoiding contams entering the growing medium.

Preparation & materials

Empty jars -  As apposed to PF Tek, the type of jars used don't really matter as long as they are convenient to work with and have good fitting screw able lids. I used four 350ml jars for this experiment.

Bee honey -  Any honey should work basically.

Plastic bread wrappers -   Between the lids and the jars, two layers of plastic are put to avoid airborne contaminants in. Any plastic wrap that can withstand the heat of a pressure cooker should do.

Pressure cooker -A simple pressure cooker will do. I got mine for around 20 Euro.

A handful of broken glass - Just smash up an old wine glass and have 2 -3 small pieces ready for each jar.